Nur77 (phospho Ser351) Polyclonal Antibody

Rabbit Anti-Nur77 (phospho Ser351) Polyclonal Antibody

Product Specifications

Background

NR4A1 encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The nuclear receptor subfamily 4 group A member 1 acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

NR4A1; GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3

Gene ID

3164

Accession Number

P22736

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Nur77 around the phosphorylation site of S351

Clonality

Polyclonal

Tag

Nuclear receptor subfamily 4 group A member 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

65-70kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Nur77 (S351) Polyclonal Antibody detects endogenous levels of Nur77 protein only when phosphorylated at S351.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200