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Histone H3.3 (phospho Ser31) Polyclonal Antibody

Rabbit Anti-Histone H3.3 (phospho Ser31) Polyclonal Antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A contains introns and its mRNA is polyadenylated, unlike most histone genes. The H3 histone family member 3A encoded is a replication-independent member of the histone H3 family.

CAS Number

9007-83-4

Product Name Alternative

H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Gene ID

3020/3021

Accession Number

P84243

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Histone H3.3 around the phosphorylation site of S31

Clonality

Polyclonal

Tag

Histone H3.3

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Histone H3.3 (S31) Polyclonal Antibody detects endogenous levels of Histone H3.3 protein only when phosphorylated at S31.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

Other Product Names

H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P84243

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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