GPR31 Polyclonal Antibody

Rabbit Anti-GPR31 Polyclonal Antibody

Product Specifications

Background

GPR31 (G Protein-Coupled Receptor 31) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. GO annotations related to this gene include G-protein coupled receptor activity. An important paralog of this gene is HCAR3. igh-affinity receptor for 12- (S) -hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE) . 12- (S) -HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.

CAS Number

9007-83-4

Product Name Alternative

GPR31; 12- (S) -hydroxy-5; 8,10,14-eicosatetraenoic acid receptor; 12- (S) -HETE receptor; 12-HETER; G-protein coupled receptor 31

Gene ID

2853

Accession Number

O00270

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human GPR31 at AA rangle: 150-230

Clonality

Polyclonal

Tag

12- (S) -hydroxy-5,8,10,14-eicosatetraenoic acid receptor

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

33kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

GPR31 Polyclonal Antibody detects endogenous levels of GPR31 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:5000; Not yet tested in other applications.