Gα i-3 Polyclonal Antibody

Rabbit Anti-Gα i-3 Polyclonal Antibody

Product Specifications

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. GNAI3 encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

CAS Number

9007-83-4

Product Name Alternative

GNAI3; Guanine nucleotide-binding protein G (k) subunit alpha; G (i) alpha-3

Gene ID

2773

Accession Number

P08754

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human Gα i-3 at AA rangle: 1-80

Clonality

Polyclonal

Tag

Guanine nucleotide-binding protein G (k) subunit alpha

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

41kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Gα i-3 Polyclonal Antibody detects endogenous levels of Gα i-3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.