Pdcd-4 (phospho Ser457) Polyclonal Antibody

Rabbit Anti-Pdcd-4 (phospho Ser457) Polyclonal Antibody

Product Specifications

Background

PDCD4 is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

PDCD4; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a

Gene ID

27250

Accession Number

Q53EL6

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Pdcd-4 around the phosphorylation site of S457

Clonality

Polyclonal

Tag

Programmed cell death protein 4

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

51kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Pdcd-4 (S457) Polyclonal Antibody detects endogenous levels of Pdcd-4 protein only when phosphorylated at S457.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200