Connexin 43 (phospho Ser261) Polyclonal Antibody

Rabbit Anti-Connexin 43 (phospho Ser261) Polyclonal Antibody

Product Specifications

Background

GJA1 is a member of the connexin gene family. The gap junction protein alpha 1 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.

CAS Number

9007-83-4

Product Name Alternative

GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein

Gene ID

2697

Accession Number

P17302

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human Connexin 43 around the phosphorylation site of S261

Clonality

Polyclonal

Tag

Gap junction alpha-1 protein

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Connexin 43 (S261) Polyclonal Antibody detects endogenous levels of Connexin 43 protein only when phosphorylated at S261.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200