Gab 1 (phospho Tyr659) Polyclonal Antibody

Rabbit Anti-Gab 1 (phospho Tyr659) Polyclonal Antibody

Product Specifications

Background

The GRB2 associated binding protein 1 encoded by GAB1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1

Gene ID

2549

Accession Number

Q13480

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Gab 1 around the phosphorylation site of Y659

Clonality

Polyclonal

Tag

GRB2-associated-binding protein 1

Applications

WB, IHC, IF, ELISA

Field of Research

Cancer#Epigenetics

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

76kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Gab 1 (Y659) Polyclonal Antibody detects endogenous levels of Gab 1 protein only when phosphorylated at Y659.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.