Fyn (phospho Tyr530) Polyclonal Antibody

Rabbit Anti-Fyn (phospho Tyr530) Polyclonal Antibody

Product Specifications

Background

FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The FYN proto-oncogene, Src family tyrosine kinase associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

CAS Number

9007-83-4

Product Name Alternative

FYN; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn

Gene ID

2534

Accession Number

P06241

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Fyn around the phosphorylation site of Y530

Clonality

Polyclonal

Tag

Tyrosine-protein kinase Fyn

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

60kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Fyn (Y530) Polyclonal Antibody detects endogenous levels of Fyn protein only when phosphorylated at Y530.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200