SIRT1 (phospho Ser47) Polyclonal Antibody

Rabbit Anti-SIRT1 (phospho Ser47) Polyclonal Antibody

Product Specifications

Background

SIRT1 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The sirtuin 1 encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

SIRT1; SIR2L1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2

Gene ID

23411

Accession Number

Q96EB6

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human SIRT1 around the phosphorylation site of S47

Clonality

Polyclonal

Tag

NAD-dependent protein deacetylase sirtuin-1

Applications

WB, ELISA, IHC

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

85-110kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-SIRT1 (S47) Polyclonal Antibody detects endogenous levels of SIRT1 protein only when phosphorylated at S47.

Observed Molecular Weight

85-110

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000