ERF (phospho Thr526) Polyclonal Antibody

Rabbit Anti-ERF (phospho Thr526) Polyclonal Antibody

Product Specifications

Background

ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by ERF binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2

Gene ID

2077

Accession Number

P50548

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human ERF around the phosphorylation site of T526

Clonality

Polyclonal

Tag

ETS domain-containing transcription factor ERF

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

58kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-ERF (T526) Polyclonal Antibody detects endogenous levels of ERF protein only when phosphorylated at T526.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.