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Eps15 (phospho Tyr849) Polyclonal Antibody

Rabbit Anti-Eps15 (phospho Tyr849) Polyclonal Antibody

Product Specifications

Background

EPS15 encodes epidermal growth factor receptor pathway substrate 15 that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, EPS15 is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Name Alternative

EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p

Gene ID

2060

Accession Number

P42566

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Eps15 around the phosphorylation site of Y849

Clonality

Polyclonal

Tag

Epidermal growth factor receptor substrate 15

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

100kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.

Product Datasheet

https://www.abbkine.com/datasheet/ABP54141.pdf

Observed Molecular Weight

100

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

EPS15; AF1P; Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P42566

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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