GRK 2 (phospho Ser685) Polyclonal Antibody

Rabbit Anti-GRK 2 (phospho Ser685) Polyclonal Antibody

Product Specifications

Background

The product of GRK2 phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. G protein-coupled receptor kinase 2 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

CAS Number

9007-83-4

Product Name Alternative

ADRBK1; BARK; BARK1; GRK2; Beta-adrenergic receptor kinase 1; Beta-ARK-1; G-protein coupled receptor kinase 2

Gene ID

156

Accession Number

P25098

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human GRK 2 around the phosphorylation site of S685

Clonality

Polyclonal

Tag

Beta-adrenergic receptor kinase 1

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

80kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-GRK 2 (S685) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S685.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:5000; Not yet tested in other applications.