Casein Kinase IIβ (phospho Ser209) Polyclonal Antibody

Rabbit Anti-Casein Kinase IIβ (phospho Ser209) Polyclonal Antibody

Product Specifications

Background

CSNK2B encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

CSNK2B; CK2N; G5A; Casein kinase II subunit beta; CK II beta; Phosvitin; Protein G5a

Gene ID

1460

Accession Number

P67870

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Casein Kinase IIβ around the phosphorylation site of S209

Clonality

Polyclonal

Tag

Casein kinase II subunit beta

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

25kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Casein Kinase IIβ (S209) Polyclonal Antibody detects endogenous levels of Casein Kinase IIβ protein only when phosphorylated at S209.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200