Acetyl Histone H1 (K25) Polyclonal Antibody

Rabbit Anti-Acetyl Histone H1 (K25) Polyclonal Antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The H1 histone family member O, oocyte specific encoded is a replication-independent histone that is a member of the histone H1 family. H1FOO contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes.

CAS Number

9007-83-4

Product Name Alternative

H1FOO; H1OO; OSH1; Histone H1oo; Oocyte-specific histone H1; Oocyte-specific linker histone H1; osH1

Gene ID

132243

Accession Number

Q8IZA3

Reactivity

Human, Monkey

Immunogen

Synthesized peptide derived from human Histone H1 around the acetylation site of K25 at AA rangle: 100-180

Clonality

Polyclonal

Tag

Histone H1oo

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

20kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Acetyl-Histone H1 (K25) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when acetylated at K25.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200