IKKα/β (phospho Ser176/177) Polyclonal Antibody

Rabbit Anti-IKKα/β (phospho Ser176/177) Polyclonal Antibody

Product Specifications

Background

CHUK encodes a member of the serine/threonine protein kinase family. The conserved helix-loop-helix ubiquitous kinase, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

CAS Number

9007-83-4

Product Name Alternative

CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B

Gene ID

1147/3551

Accession Number

O15111/O14920

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human IKKα/β around the phosphorylation site of S176/177

Clonality

Polyclonal

Tag

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

80kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-IKKα/β (S176/177) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S176/177.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200