Chk1 (phospho Ser345) Polyclonal Antibody

Rabbit Anti-Chk1 (phospho Ser345) Polyclonal Antibody

Product Specifications

Background

The checkpoint kinase 1 encoded by CHEK1 belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1

Gene ID

1111

Accession Number

O14757

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Chk1 around the phosphorylation site of S345

Clonality

Polyclonal

Tag

Serine threonine-protein kinase Chk1

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Chk1 (S345) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S345.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200