ME3 Polyclonal Antibody

Rabbit Anti-ME3 Polyclonal Antibody

Product Specifications

Background

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP (+) -dependent isoform, a mitochondrial NADP (+) -dependent isoform, and a mitochondrial NAD (+) -dependent isoform. This gene encodes a mitochondrial NADP (+) -dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

CAS Number

9007-83-4

Product Name Alternative

ME3; NADP-dependent malic enzyme; mitochondrial; NADP-ME; Malic enzyme 3

Gene ID

10873

Accession Number

Q16798

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the C-terminal region of human ME3 at AA rangle: 520-600

Clonality

Polyclonal

Tag

NADP-dependent malic enzyme mitochondrial

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

67kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

ME3 Polyclonal Antibody detects endogenous levels of ME3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200