DNAM-1 (phospho Ser329) Polyclonal Antibody

Rabbit Anti-DNAM-1 (phospho Ser329) Polyclonal Antibody

Product Specifications

Background

CD226 encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The CD226 molecule mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

CD226; DNAM1; CD226 antigen; DNAX accessory molecule 1; DNAM-1; CD antigen CD226

Gene ID

10666

Accession Number

Q15762

Reactivity

Human, Mouse, Monkey

Immunogen

Synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

Clonality

Polyclonal

Tag

CD226 antigen

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

60kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-DNAM-1 (S329) Polyclonal Antibody detects endogenous levels of DNAM-1 protein only when phosphorylated at S329.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.