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CENP-A Polyclonal Antibody

Rabbit Anti-CENP-A Polyclonal Antibody

Product Specifications

Background

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4) 2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Product Name Alternative

CENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A

Gene ID

1058

Accession Number

P49450

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the N-terminal region of human CENP-A at AA rangle: 30-110

Clonality

Polyclonal

Tag

Histone H3-like centromeric protein A

Applications

IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

CENP-A Polyclonal Antibody detects endogenous levels of CENP-A protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200

Other Product Names

CENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P49450

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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