P57 (phospho Thr310) Polyclonal Antibody

Rabbit Anti-p57 (phospho Thr310) Polyclonal Antibody

Product Specifications

Background

CDKN1C is imprinted, with preferential expression of the maternal allele. The cyclin dependent kinase inhibitor 1C is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that CDKN1C is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

CDKN1C; KIP2; Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2

Gene ID

1028

Accession Number

P49918

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human p57 around the phosphorylation site of T310

Clonality

Polyclonal

Tag

Cyclin-dependent kinase inhibitor 1C

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

32kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-p57 (T310) Polyclonal Antibody detects endogenous levels of p57 protein only when phosphorylated at T310.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.