Coelenteramine 400a

Coelenteramine 400a (Coelenterazine 400a), a derivative of Coelenterazine, is a Renilla luciferase (RLuc) substrate. In the presence of Coelenteramine 400a, RLuc can emit blue light at 395 nm[1][2]. Coelenterazine 400a will causes color change in the bioluminescence reaction of Rluc by replacing the sulfur and oxygen heteroatoms of the methylene bridge. Coelenterazine 400a provides higher signal resolution and can be used in the research of bioluminescence resonance energy transfer (BRET) [3].

Product Specifications

CAS Number

[70217-82-2]

Product Name Alternative

Coelenterazine 400a; Bisdeoxycoelenterazine

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/coelenteramine-400a.html

Purity

99.70

Solubility

DMSO : 8.33 mg/mL (ultrasonic) |Ethanol : 1 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C1C(CC2=CC=CC=C2)=NC3=C(NC(C4=CC=CC=C4)=CN31)CC5=CC=CC=C5

Molecular Formula

C26H21N3O

Molecular Weight

391.46

Precautions

H302, H315, H319, H335

References & Citations

[1]Levi J, et, al. Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays. J Am Chem Soc. 2007 Oct 3;129 (39) :11900-1. |[2]Bertrand L, et, al. The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS) . J Recept Signal Transduct Res. Feb-Nov 2002;22 (1-4) :533-41.|[3]Yuan M L, et al. Luminescence of coelenterazine derivatives with C-8 extended electronic conjugation[J]. Chinese Chemical Letters, 2016, 27 (4) : 550-554.|[4]Ikeda Y, et al. Quantitative evaluation of luminescence intensity from enzymatic luminescence reaction of coelenterazine and analogues. Journal of Photochemistry and Photobiology A: Chemistry, 2020, 394: 112459.