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Btk Polyclonal Antibody

Rabbit Anti-Btk Polyclonal Antibody

Product Specifications

Background

Tyrosine-protein kinase BTK encoded by BTK plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Product Name Alternative

BTK; AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase

Gene ID

695

Accession Number

Q06187

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Btk around the non-phosphorylation site of Y551

Clonality

Polyclonal

Tag

Tyrosine-protein kinase BTK

Applications

WB, IHC, IF, ELISA

Field of Research

Signal Transduction

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

76kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Btk Polyclonal Antibody detects endogenous levels of Btk protein only when non-phosphorylation at Y550.

Product Datasheet

https://www.abbkine.com/datasheet/ABP53077.pdf

Observed Molecular Weight

76

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

Other Product Names

BTK; AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/Q06187

Isotype

Rabbit IgG

Available Sizes

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