BNIP-3 Polyclonal Antibody

Rabbit Anti-BNIP-3 Polyclonal Antibody

Product Specifications

Background

BNIP3 is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The BCL2 interacting protein 3 interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.

CAS Number

9007-83-4

Product Name Alternative

BNIP3; NIP3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Gene ID

664

Accession Number

Q12983

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human BNIP-3

Clonality

Polyclonal

Tag

BCL2

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

22kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

BNIP-3 Polyclonal Antibody detects endogenous levels of BNIP-3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200