Myosin VI Polyclonal Antibody

Rabbit Anti-Myosin VI Polyclonal Antibody

Product Specifications

Background

MYO6 encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The unconventional myosin-VI consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Product Name Alternative

MYO6; KIAA0389; Unconventional myosin-VI; Unconventional myosin-6

Gene ID

4646

Accession Number

Q9UM54

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human Myosin VI at AA range: 40-120

Clonality

Polyclonal

Tag

Unconventional myosin-VI

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

149kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Myosin VI Polyclonal Antibody detects endogenous levels of Myosin VI protein.

Observed Molecular Weight

149

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:5000; IF 1:50-200