WTAP Polyclonal Antibody

Rabbit Anti-WTAP Polyclonal Antibody

Product Specifications

Background

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. WTAP encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, pre-mRNA-splicing regulator WTAP is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.

CAS Number

9007-83-4

Product Name Alternative

WTAP; KIAA0105; Pre-mRNA-splicing regulator WTAP; Female-lethal (2) D homolog; hFL (2) D; WT1-associated protein; Wilms tumor 1-associating protein

Gene ID

9589

Accession Number

Q15007

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the C-terminal region of human WTAP at AA range: 290-370

Clonality

Polyclonal

Tag

Pre-mRNA-splicing regulator WTAP

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

55kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

WTAP Polyclonal Antibody detects endogenous levels of WTAP protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200