UBF-1 Polyclonal Antibody

Rabbit Anti-UBF-1 Polyclonal Antibody

Product Specifications

Background

UBTF encodes a member of the HMG-box DNA-binding protein family. The nucleolar transcription factor 1 plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11.

CAS Number

9007-83-4

Product Name Alternative

UBTF; UBF; UBF1; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1

Gene ID

7343

Accession Number

P17480

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human UBF-1 at AA range: 470-550

Clonality

Polyclonal

Tag

Nucleolar transcription factor 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

90kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

UBF-1 Polyclonal Antibody detects endogenous levels of UBF-1 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200