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Stat5 Polyclonal Antibody

Rabbit Anti-Stat5 Polyclonal Antibody

Product Specifications

Background

Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation. This phosphorylation is mediated by Src upon erythropoietin stimulation. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells.

Product Name Alternative

STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B

Gene ID

6776/6777

Accession Number

P42229/P51692

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Stat5 around the non-phosphorylation site of Y694/699

Clonality

Polyclonal

Tag

Signal transducer and activator of transcription 5A

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

91kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Stat5 Polyclonal Antibody detects endogenous levels of Stat5 protein.

Product Datasheet

https://www.abbkine.com/datasheet/ABP52519.pdf

Observed Molecular Weight

91

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200

Other Product Names

STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P42229

CAS Number

9007-83-4

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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