SMG7 Polyclonal Antibody

Rabbit Anti-SMG7 Polyclonal Antibody

Product Specifications

Background

SMG7 encodes protein SMG7 that is essential for nonsense-mediated mRNA decay (NMD) ; a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Product Name Alternative

SMG7; C1orf16; EST1C; KIAA0250; Protein SMG7; EST1-like protein C; SMG-7 homolog; hSMG-7

Gene ID

9887

Accession Number

Q92540

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human SMG7 at AA range: 490-570

Clonality

Polyclonal

Tag

Protein SMG7

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

127kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

SMG7 Polyclonal Antibody detects endogenous levels of SMG7 protein.

Observed Molecular Weight

127

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.