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Rad18 Polyclonal Antibody

Rabbit Anti-Rad18 Polyclonal Antibody

Product Specifications

Background

RAD18, E3 ubiquitin protein ligase encoded by RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18, E3 ubiquitin protein ligase is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

CAS Number

9007-83-4

Product Name Alternative

RAD18; RNF73; E3 ubiquitin-protein ligase RAD18; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73

Gene ID

56852

Accession Number

Q9NS91

Reactivity

Human, Rat

Immunogen

Synthesized peptide derived from the Internal region of human Rad18 at AA range: 270-350

Clonality

Polyclonal

Tag

E3 ubiquitin-protein ligase RAD18

Applications

WB, IHC

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

56kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Rad18 Polyclonal Antibody detects endogenous levels of Rad18 protein.

Product Datasheet

https://www.abbkine.com/datasheet/ABP52305.pdf

Observed Molecular Weight

56

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300

Other Product Names

RAD18; RNF73; E3 ubiquitin-protein ligase RAD18; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/Q9NS91

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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