Rac1/2/3/CDC42 Polyclonal Antibody

Rabbit Anti-Rac1/2/3/CDC42 Polyclonal Antibody

Product Specifications

Background

Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) encoded by RAC1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for RAC1.

CAS Number

9007-83-4

Product Name Alternative

RAC1; TC25; MIG5; Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1; RAC2; Ras-related C3 botulinum toxin substrate 2; GX; Small G protein; p21-Rac2; RAC3; Ras-related C3 bot

Gene ID

5879/5880/5881/998

Accession Number

P63000/P15153/P60763/P60953

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Rac1/2/3/CDC42 around the non-phosphorylation site of S71

Clonality

Polyclonal

Tag

Ras-related C3 botulinum toxin substrate 3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

26kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Rac1/2/3/CDC42 Polyclonal Antibody detects endogenous levels of Rac1/2/3/CDC42 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200