Rac GAP1 Polyclonal Antibody

Rabbit Anti-Rac GAP1 Polyclonal Antibody

Product Specifications

Background

RACGAP1 encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. Rac GTPase activating protein 1 binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. Rac GTPase activating protein 1 plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for RACGAP1. There is a pseudogene for RACGAP1 on chromosome 12.

CAS Number

9007-83-4

Product Name Alternative

RACGAP1; KIAA1478; MGCRACGAP; Rac GTPase-activating protein 1; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolg; CYK4; HsCYK-4

Gene ID

29127

Accession Number

Q9H0H5

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human Rac GAP1 around the non-phosphorylation site of S387

Clonality

Polyclonal

Tag

Rac GTPase-activating protein 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

72kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Rac GAP1 Polyclonal Antibody detects endogenous levels of Rac GAP1 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.