PIAS 3 Polyclonal Antibody

Rabbit Anti-PIAS 3 Polyclonal Antibody

Product Specifications

Background

PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription) ] family of transcriptional modulators. Protein inhibitor of activated STAT 3 functions as a SUMO (small ubiquitin-like modifier) -E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of PIAS3 have been identified, but the full-length nature of some of these variants has not been determined.

CAS Number

9007-83-4

Product Name Alternative

PIAS3; E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3

Gene ID

10401

Accession Number

Q9Y6X2

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human PIAS 3 at AA range: 10-90

Clonality

Polyclonal

Tag

E3 SUMO-protein ligase PIAS3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

68kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

PIAS 3 Polyclonal Antibody detects endogenous levels of PIAS 3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200