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PDHA1 Polyclonal Antibody

Rabbit Anti-PDHA1 Polyclonal Antibody

Product Specifications

Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. PDHA1 encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in PDHA1 are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for PDHA1.

CAS Number

9007-83-4

Product Name Alternative

PDHA1; PHE1A; Pyruvate dehydrogenase E1 component subunit alpha; somatic form, mitochondrial; PDHE1-A type I

Gene ID

5160

Accession Number

P08559

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the C-terminal region of human PDHA1 at AA range: 290-370

Clonality

Polyclonal

Tag

Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

43kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

PDHA1 Polyclonal Antibody detects endogenous levels of PDHA1 protein.

Product Datasheet

https://www.abbkine.com/datasheet/ABP52168.pdf

Observed Molecular Weight

43

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200

Other Product Names

PDHA1; PHE1A; Pyruvate dehydrogenase E1 component subunit alpha; somatic form, mitochondrial; PDHE1-A type I

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P08559

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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