Nanog P8 Polyclonal Antibody

Rabbit Anti-Nanog P8 Polyclonal Antibody

Product Specifications

Background

This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG.

CAS Number

9007-83-4

Product Name Alternative

NANOGP8; Putative homeobox protein NANOGP8

Gene ID

388112

Accession Number

Q6NSW7

Reactivity

Human

Immunogen

Synthesized peptide derived from the Internal region of human Nanog P8 at AA range: 30-110

Clonality

Polyclonal

Tag

Putative homeobox protein NANOGP8

Applications

WB, IF, ELISA, IHC

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

35kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Nanog P8 Polyclonal Antibody detects endogenous levels of Nanog P8 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IF/ICC1:50-200; ELISA 1:2000-20000; IHC 1:50-200