MLH1 Polyclonal Antibody

Rabbit Anti-MLH1 Polyclonal Antibody

Product Specifications

Background

MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . It is a human homolog of the E.coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.

CAS Number

9007-83-4

Product Name Alternative

MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1

Gene ID

4292

Accession Number

P40692

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human MLH1 at AA range: 410-490

Clonality

Polyclonal

Tag

DNA mismatch repair protein Mlh1

Applications

WB, IHC

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

85kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

MLH1 Polyclonal Antibody detects endogenous levels of MLH1 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300