Histone 1.0 Polyclonal Antibody

Rabbit Anti-Histone 1.0 Polyclonal Antibody

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H1F0 is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

CAS Number

9007-83-4

Product Name Alternative

H1F0; H1FV; Histone H1.0; Histone H1'; Histone H1 (0)

Gene ID

3005

Accession Number

P07305

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human Histone 1.0 at AA range: 40-120

Clonality

Polyclonal

Tag

Histone H1.0

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

28kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Histone 1.0 Polyclonal Antibody detects endogenous levels of Histone 1.0 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200