Frizzled-7 Polyclonal Antibody

Rabbit Anti-Frizzled-7 Polyclonal Antibody

Product Specifications

Background

Members of the frizzled gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Frizzled-7 contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.

CAS Number

9007-83-4

Product Name Alternative

FZD7; Frizzled-7; Fz-7; hFz7; FzE3

Gene ID

8324

Accession Number

O75084

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the N-terminal region of human Frizzled-7 at AA range: 20-100

Clonality

Polyclonal

Tag

Frizzled-7

Applications

WB, Flow Cyt, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

64kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; Flow Cyt 1:50-200; IF/ICC1:100-500; ELISA 1:5000-20000