Fli-1 Polyclonal Antibody

Rabbit Anti-Fli-1 Polyclonal Antibody

Product Specifications

Background

FLI1 encodes friend leukemia integration 1 transcription factor containing an ETS DNA-binding domain. FLI1 can undergo a t (11;22) (q24; q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4;11) (q21; q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

FLI1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB

Gene ID

2313

Accession Number

Q01543

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human Fli-1 at AA range: 260-340

Clonality

Polyclonal

Tag

Friend leukemia integration 1 transcription factor

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

50kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Fli-1 Polyclonal Antibody detects endogenous levels of Fli-1 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200