Fhit Polyclonal Antibody

Rabbit Anti-Fhit Polyclonal Antibody

Product Specifications

Background

FHIT, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1, P3-triphosphate hydrolase involved in purine metabolism. FHIT encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of FHIT. In fact, aberrant transcripts from FHIT have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for FHIT.

CAS Number

9007-83-4

Product Name Alternative

FHIT; Bis (5'-adenosyl) -triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1, P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

Gene ID

2272

Accession Number

P49789

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human Fhit at AA range: 60-140

Clonality

Polyclonal

Tag

Bis (5'-adenosyl) -triphosphatase

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

16kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Fhit Polyclonal Antibody detects endogenous levels of Fhit protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

FHIT; Bis (5'-adenosyl) -triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1, P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein