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DNA Ligase III Polyclonal Antibody

Selected Variant

ABP51183-01

Size: 30 µL

Rabbit Anti-DNA Ligase III Polyclonal Antibody

Product Specifications

Background

LIG3 is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. DNA ligase 3 encoded by LIG3 is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

CAS Number

9007-83-4

Product Name Alternative

LIG3; DNA ligase 3; DNA ligase III; Polydeoxyribonucleotide synthase [ATP] 3

Gene ID

3980

Accession Number

P49916

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human DNA Ligase III at AA range: 110-190

Clonality

Polyclonal

Tag

DNA ligase 3

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

100kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

DNA Ligase III Polyclonal Antibody detects endogenous levels of DNA Ligase III protein.

Observed Molecular Weight

100

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

LIG3; DNA ligase 3; DNA ligase III; Polydeoxyribonucleotide synthase [ATP] 3

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P49916

Isotype

Rabbit IgG

Available Sizes

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