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DNA Ligase I Polyclonal Antibody

Rabbit Anti-DNA Ligase I Polyclonal Antibody

Product Specifications

Background

LIG1 encodes a member of the ATP-dependent DNA ligase protein family. DNA ligase 1 functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of LIG1 may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

LIG1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1

Gene ID

3978

Accession Number

P18858

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from the N-terminal region of human DNA Ligase I at AA range: 80-160

Clonality

Polyclonal

Tag

DNA ligase 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

133kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein.

Observed Molecular Weight

133

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

Other Product Names

LIG1; DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P18858

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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