Creatine Kinase M Polyclonal Antibody

Rabbit Anti-Creatine Kinase M Polyclonal Antibody

Product Specifications

Background

Creatine kinase, M-typeencoded by CKM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

CAS Number

9007-83-4

Product Name Alternative

CKM; CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK

Gene ID

1158

Accession Number

P06732

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human Creatine Kinase M at AA range: 10-90

Clonality

Polyclonal

Tag

Creatine kinase M-type

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

43kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Creatine Kinase M Polyclonal Antibody detects endogenous levels of Creatine Kinase M protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.