What is the catalog number for Anti-Beclin 1 Antibody?

The catalog number for Anti-Beclin 1 Antibody is 23010-1.

What sizes are available for Anti-Beclin 1 Antibody?

Anti-Beclin 1 Antibody is available in the following size(s): 100 µg.

Is Anti-Beclin 1 Antibody in stock?

Anti-Beclin 1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

Anti-Beclin 1 Antibody

Rabbit Polyclonal Antibody specific to Beclin1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Coiled-coil myosin-like BCL2-interacting protein, Protein GT197 [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa]

Gene Name

BECN1

NCBI Gene ID

UniProt

Q14457

Cellular Locus

Cytoplasm, Golgi apparatus, trans-Golgi network membrane, Endosome membrane, Endoplasmic reticulum membrane, Mitochondrion membrane, Endosome, Cytoplasmic vesicle, autophagosome

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids at the C-terminus of human Beclin 1.

Target Antigen

Beclin-1

Target

Beclin 1

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Autophagy

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. {PubMed:18570871, PubMed:20208530, PubMed:20643123, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25275521, PubMed:26783301, PubMed:28445460, PubMed:9765397, PubMed:21364619, PubMed:26263979}.

Additionnal Information

Immunoblotting: use at 1- 2ug/mL. A band of ~51kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human Beclin 1.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml

Buffer pH

pH 7.4

Target Background

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Beclin 1, the mammalian orthologue of yeast Atg6, has a central role in autophagy. It interacts with several cofactors (Atg14L, UVRAG, Bif-1, Rubicon, Ambra1, HMGB1, nPIST, VMP1, SLAM, IP3R, PINK and survivin) to regulate the lipid kinase Vps-34 protein and promote formation of Beclin 1-Vps34-Vps15 core complexes, thereby inducing autophagy. Beclin 1 dysfunction has been implicated in many disorders including cancer and neurodegeneration.