Actinin-α3 Polyclonal Antibody

Rabbit Anti-Actinin-α3 Polyclonal Antibody

Product Specifications

Background

ACTN3 encodes a member of the alpha-actin binding protein gene family. Actinin alpha 3 (gene/pseudogene) is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of ACTN3 is associated with elite athlete status.

CAS Number

9007-83-4

Product Name Alternative

ACTN3; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein

Gene ID

Accession Number

Q08043

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human Actinin-α3 at AA range: 10-90

Clonality

Polyclonal

Tag

Alpha-actinin-3

Applications

WB, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

103kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Actinin-α3 Polyclonal Antibody detects endogenous levels of Actinin-α3 protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.