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Actin α1 Polyclonal Antibody

Rabbit Anti-Actin α1 Polyclonal Antibody

Product Specifications

Background

Actin, alpha 1, skeletal muscle encoded by ACTA1 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

CAS Number

9007-83-4

Product Name Alternative

ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1

Gene ID

58

Accession Number

P68133

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human Actin α1 at AA range: 1-80

Clonality

Polyclonal

Tag

Actin alpha skeletal muscle

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

45kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Actin α1 Polyclonal Antibody detects endogenous levels of Actin α1 protein.

Product Datasheet

https://www.abbkine.com/datasheet/ABP50591.pdf

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

Other Product Names

ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P68133

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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