14-3-3 ζ Polyclonal Antibody

Rabbit Anti-14-3-3 ζ Polyclonal Antibody

Product Specifications

Background

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for YWHAZ.

CAS Number

9007-83-4

Product Name Alternative

YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

Gene ID

7534

Accession Number

P63104

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human 14-3-3 ζ around the non-phosphorylation site of S58

Clonality

Polyclonal

Tag

14-3-3 protein zeta

Applications

WB, IHC, IP, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

28kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

14-3-3 ζ Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IP 2-5 ug:mg lysate; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.