HNF4-α (phospho Ser313) Polyclonal Antibody

Rabbit Anti-HNF4-α (phospho Ser313) Polyclonal Antibody

Product Specifications

Background

Hepatocyte nuclear factor 4 alpha encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. Hepatocyte nuclear factor 4 alpha controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms.

CAS Number

9007-83-4

Product Name Alternative

HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4

Gene ID

3172

Accession Number

P41235

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human HNF4-α around the phosphorylation site of S313

Clonality

Polyclonal

Tag

Hepatocyte nuclear factor 4-alpha

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

52kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-HNF4-α (S313) Polyclonal Antibody detects endogenous levels of HNF4-α protein only when phosphorylated at S313.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

Other Product Names

HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4