Caldesmon (phospho Ser789) Polyclonal Antibody

Rabbit Anti-Caldesmon (phospho Ser789) Polyclonal Antibody

Product Specifications

Background

CALD1 encodes a calmodulin- and actin-binding protein (caldesmon 1) that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca (2+) -calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca (2+) - dependent inhibition of smooth muscle contraction. Alternative splicing of CALD1 results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Product Name Alternative

CALD1; CAD; CDM; Caldesmon; CDM

Gene ID

800

Accession Number

Q05682

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human Caldesmon around the phosphorylation site of S789

Clonality

Polyclonal

Tag

Caldesmon

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

80kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-Caldesmon (S789) Polyclonal Antibody detects endogenous levels of Caldesmon protein only when phosphorylated at S789.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.