CREB-1 (phospho Ser142) Polyclonal Antibody

Rabbit Anti-CREB-1 (phospho Ser142) Polyclonal Antibody

Product Specifications

Background

CREB1 encodes a transcription factor (cAMP responsive element binding protein 1) that is a member of the leucine zipper family of DNA binding proteins. cAMP responsive element binding protein 1 binds as a homodimer to the cAMP-responsive element, an octameric palindrome. cAMP responsive element binding protein 1 is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of CREB1 results in several transcript variants encoding different isoforms.

CAS Number

9007-83-4

Product Name Alternative

CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1

Gene ID

1385

Accession Number

P16220

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human CREB-1 around the phosphorylation site of S142

Clonality

Polyclonal

Tag

Cyclic AMP-responsive element-binding protein 1

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

37kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-CREB-1 (S142) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S142.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.