YAP (phospho Ser127) Polyclonal Antibody

Rabbit Anti-YAP (phospho Ser127) Polyclonal Antibody

Product Specifications

Background

YAP1 encodes a downstream nuclear effector (Yes associated protein 1) of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. YAP1 is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Product Name Alternative

YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65

Gene ID

10413

Accession Number

P46937

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from human YAP around the phosphorylation site of S127

Clonality

Polyclonal

Tag

Yorkie homolog

Applications

IF, WB, IHC, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

65kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-YAP (S127) Polyclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at S127.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; Not yet tested in other applications